Humanin

⚠️ Structural Separation Notice

The Ageless Pep Academy is a clinical education property independent from any commerce operation. Any references in this profile to Ageless Peps product SKUs, pricing, or the agelesspeps.com domain are for completeness and transparency; they are not endorsements and do not form part of the clinical education content. Clinicians are responsible for independent verification of any product sourcing decision. The Academy's Medical Director provides editorial oversight only and does not endorse commercial products.

Quick Facts

• Full Name: Humanin (HN)
• Aliases: HN, Humanin-G (HNG — potent glycine-14 analog)
• Category: Longevity / Mitochondrial Health / Neuroprotection
• Sequence: 21 amino acids (mitochondrial-derived peptide, encoded in 16S rRNA region)
• Molecular Weight: ~2.8 kDa
• Administration: Subcutaneous / Intranasal (research)
• Typical Dose: 2–4 mg/week SubQ (human extrapolation; doses vary widely)
• Half-Life: Short; multiple dosing strategies explored
• Status: Research Only
• WADA Status: Not currently listed

What It Does

Humanin is a small peptide encoded within the mitochondrial genome (16S rRNA region), making it one of a class called mitochondrial-derived peptides (MDPs). It is cytoprotective across multiple tissues — brain, heart, vasculature, and metabolic organs. Humanin levels decline with age and are inversely correlated with Alzheimer's disease risk and metabolic dysfunction. It is considered a key longevity signaling molecule and a bridge between mitochondrial health and systemic aging.

Mechanism of Action

1. Anti-apoptotic signaling: Activates JAK2/STAT3, PI3K/Akt, and MAPK pathways → inhibits Bax-mediated mitochondrial apoptosis
2. Neuronal protection: Blocks amyloid-beta (Aβ) toxicity in neurons; reduces tau pathology markers
3. Insulin sensitization: Activates FPRL1/FPR2 and gp130 receptors → improves insulin sensitivity and glucose metabolism
4. Mitochondrial function: Supports mitochondrial membrane potential and reduces ROS production
5. IGF-1 modulation: Interacts with IGFBP-3, modulating IGF-1 bioavailability and longevity signaling
6. Systemic cytoprotection: Cardioprotective, hepatoprotective, and vasculoprotective effects reported

Ageless Peps Products

• APWS-Humanin-Vial — Humanin 10mg Vial, $60, wholesale only

Dosing Protocols

Research-Informed Protocol

• Dose: 2–4 mg/week SubQ (divided doses)
• Frequency: 2–3x per week
• Duration: Ongoing cycles with periodic assessment
• Note: Human dosing extrapolated from rodent studies; clinical protocols not yet established
• Source: The Peptides Bible, longevity research literature

Stacks Well With

• TB-500 – systemic cytoprotection and tissue repair synergy
• Melanotan II – complementary mitochondrial and metabolic signaling
• MOTS-C – fellow mitochondrial-derived peptide; complementary longevity signaling
• Epitalon – anti-aging and telomere support synergy
• SS-31 – mitochondrial membrane protector; powerful MDP stack

Use Cases

• Longevity and healthy aging (MDP decline reversal)
• Neuroprotection (Alzheimer's risk reduction, neuronal survival)
• Metabolic Health (insulin sensitization, glucose metabolism)
• Cardiovascular Protection
• Mitochondrial Health optimization
• Cognitive Function maintenance

Contraindications & Safety

• Active malignancy — anti-apoptotic signaling; theoretical concern
• Limited human data — extrapolated from rodent studies; use cautiously
• Drug interactions — JAK/STAT pathway involvement; caution with immunosuppressants
• Common side effects: Not well characterized in humans; generally well-tolerated in animal models
• Long-term safety profile in humans not established

Storage

• Lyophilized: -20°C long-term; 2–8°C up to 3 months
• Reconstituted: 2–8°C for 1–2 weeks; protect from light
• Solvent: Sterile/bacteriostatic water

PubMed Research

Research links will be added after PubMed search

Sources

• The Peptides Bible – Mitochondrial peptides section
• Peptide Protocols Master Bible – Longevity protocols
• Longevity research literature (Cohen et al., 2013; Bhanu et al.)

#longevity #mitochondrial #neuroprotection #cytoprotection #aging #humanin #MDP #peptide